The Hyb setup tab on the Submit Microarray Hybridization Request window is used to enter the basic general settings for the microarray hybridizations that are being requested.

These settings will be used to generate a number of default hybridization records which you will then be able to edit for specific differences that might occur in the hybridizations of individual samples.

On the tab, there are a total of six fields (counting the pair of radio buttons as a single field) under the heading Define Hybridizations.

The first field (A. Select an organism) is automatically filled in with the organism selected when describing your samples, since those samples are the ones for which you are defining the hybridizations, so it follows naturally that the type of organism for the hybridizations would be the same as the organism from which the samples were collected. (If, for some reason, you want to choose a different organism, this drop-down field is editable.)

The next field (B. Select a category of microarray) is used to indicate the kind of microarray hybridization that is to be performed from among the options presented in the drop-down list. Third are a pair of radio buttons (C. Select one of the following) in which you indicate whether the microarray is to be a standard Catalog microarray or a Custom microarray. As a default, Catalog microarray is selected. The fourth field (D. Select the microarray slide or slide set to be used for these hybridizations) as its label suggests, is used to specify the slide or slide set to be used in the hybridizations. This field will not have any options available in it until you have already selected the microarray category (part B) since, of course, the kinds of slides you can use depends on the kind of microarray hybridization to be performed, so as is often the case throughout this process, you should take each field and tab in the indicated order. Some of the selections presented are actually sets of slides, and in these cases, if you choose one of those options, the number of slides per set will be displayed to the right of the field.

The fifth field is for entering the number of hybridizations to be performed (E. How many hybridizations will you be requesting?). The value in this field (in concert with part D above in certain cases) controls how many individual hybridization records will be created on the Hybs tab when it is first accessed. Finally, the last field (F. Where will the slides come from?) is for indicating the source of the slides to be used. In most cases, the slides will be provided from the HCI Microarray Core's stock, so this is selected as the default value (Core Supplied). If your lab will provide the slides, select Client Supplied. If it is appropriate to reuse old slides from previous experiments, select Reuse Stripped Slides.

All of the fields on this tab are required. Once they have been filled in, the Next > button at the bottom center of the tab will become active.

Clicking on this button will automatically select the Hybs tab. The < Back button to the left of the Next > button automatically moves back to the Samples tab.

The Cancel button in the lower right corner of the window closes the window without submitting your hybridization request.

If you click on this button, all of the data entered on the window will be lost. Before closing the window, however, a dialog opens to verify that you really want to cancel your request.

Click Yes on this dialog to verify that you really want to close the Submit Microarray Hybridization Request window without submitting a request or No to return to the window and continue with your submission.

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